Hotspot

trnL-UAG-rpl32

Genome · 138806-139310 · 505 bp

Back to Gaultheria

Pi 0.00601

MarkerSeek score 61.40

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: Genome
length_bp: 505
variable_sites: 3
pi: 0.00601
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GAAATCCCTTTCGACCGGAA	50.0	50.0	58.0	57.9	628-634 bp	1.0	80.3
trnL-UAG-rpl32_p2	AGCGTGTCTACCGATTTCAC	TCCCTTTCGACCGGAAATTC	50.0	50.0	58.0	57.9	624-630 bp	1.0	80.3
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	GAAATCCCTTTCGACCGGAA	50.0	50.0	58.0	57.9	629-635 bp	1.0	80.2
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	TCCCTTTCGACCGGAAATTC	50.0	50.0	58.0	57.9	625-631 bp	1.0	80.2
trnL-UAG-rpl32_p5	AGCGTGTCTACCGATTTCAC	CCCTTTCGACCGGAAATTCA	50.0	50.0	58.0	58.2	623-629 bp	1.0	79.6