Hotspot

ycf3-trnK-UUU

Genome · 103279-104736 · 1458 bp

Back to Gaultheria

Pi 0.00823

MarkerSeek score 49.80

Primer available yes

Label ycf3-trnK-UUU

Feature Metadata

label_name: ycf3-trnK-UUU
feature_type: igs
region: Genome
length_bp: 1458
variable_sites: 12
pi: 0.00823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnK-UUU_p1	CTGAGCCGTATGAGGTAGGA	AACCCAACCGCTCTTTTGAT	55.0	45.0	58.0	58.0	2221-2222 bp	1.0	80.3
ycf3-trnK-UUU_p2	CTGAGCCGTATGAGGTAGGA	ACCCAACCGCTCTTTTGATT	55.0	45.0	58.0	58.0	2220-2221 bp	1.0	80.3
ycf3-trnK-UUU_p3	GAACAGGCCATTCTACAGGG	AACCCAACCGCTCTTTTGAT	55.0	45.0	58.0	58.0	2146-2147 bp	1.0	80.1
ycf3-trnK-UUU_p4	GAACAGGCCATTCTACAGGG	ACCCAACCGCTCTTTTGATT	55.0	45.0	58.0	58.0	2145-2146 bp	1.0	80.1
ycf3-trnK-UUU_p5	GGAACAGGCCATTCTACAGG	ACCCAACCGCTCTTTTGATT	55.0	45.0	58.0	58.0	2146-2147 bp	1.0	80.1