Hotspot

atpB-rbcL

Genome · 92092-92891 · 800 bp

Back to Gaultheria

Pi 0.00875

MarkerSeek score 61.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 800
variable_sites: 7
pi: 0.00875
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCCCAGGACCAGAAGGAATA	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	883 bp	1.0	71.6
atpB-rbcL_p2	GGACGGGACCAATGATTTGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	940 bp	1.0	71.5
atpB-rbcL_p3	AGGACGGGACCAATGATTTG	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	941 bp	1.0	71.4
atpB-rbcL_p4	CCCCAGGACCAGAAGGAATA	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	882 bp	1.0	71.4
atpB-rbcL_p5	GGACGGGACCAATGATTTGA	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	939 bp	1.0	71.2