Hotspot

psbM-trnD-GUC

Genome · 81014-82554 · 1541 bp

Back to Gaultheria

Pi 0.00649

MarkerSeek score 62.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1541
variable_sites: 10
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1652-1657 bp	1.0	58.8
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1653-1658 bp	1.0	54.7
psbM-trnD-GUC_p3	CGGAATTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.1	58.5	1659-1664 bp	1.0	53.1
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	1651-1656 bp	1.0	52.8
psbM-trnD-GUC_p5	CGGAATTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.1	58.5	1659-1664 bp	1.0	51.9