Hotspot

rps12-trnC-GCA

Genome · 77208-78843 · 1636 bp

Back to Gaultheria

Pi 0.0055

MarkerSeek score 61.70

Primer available yes

Label rps12-trnC-GCA

Feature Metadata

label_name: rps12-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1636
variable_sites: 9
pi: 0.0055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnC-GCA_p1	CGGGGGATTTCCTGACATTT	TTAGGCGACACCCAGATTTG	50.0	50.0	57.9	57.9	1776-1777 bp	1.0	78.4
rps12-trnC-GCA_p2	GTACATCTTCCTCGACGCTG	TTAGGCGACACCCAGATTTG	55.0	50.0	58.2	57.9	1813-1814 bp	1.0	78.2
rps12-trnC-GCA_p3	CGGGGGATTTCCTGACATTT	TTGTTGATTAGGCGACACCC	50.0	50.0	57.9	58.2	1783-1784 bp	1.0	77.8
rps12-trnC-GCA_p4	GTACATCTTCCTCGACGCTG	TTGTTGATTAGGCGACACCC	55.0	50.0	58.2	58.2	1820-1821 bp	1.0	77.6
rps12-trnC-GCA_p5	AGTACATCTTCCTCGACGCT	TTAGGCGACACCCAGATTTG	50.0	50.0	58.2	57.9	1814-1815 bp	1.0	77.6