Hotspot

atpI-trnfM-CAU

Genome · 39293-39711 · 419 bp

Back to Gaultheria

Pi 0.012
MarkerSeek score 62.10
Primer available yes
Label atpI-trnfM-CAU

Feature Metadata

label_name
atpI-trnfM-CAU
feature_type
igs
region
Genome
length_bp
419
variable_sites
5
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-trnfM-CAU_p1 TTTTGCAACTTTAGCTGCGG AAGGTTATGAGCCTTGCGAG 45.0 50.0 57.9 58.0 513-514 bp 1.0 79.6
atpI-trnfM-CAU_p2 TTTTGCAACTTTAGCTGCGG TTGCGAGCTACCAGACTACT 45.0 50.0 57.9 58.2 500-501 bp 1.0 78.6
atpI-trnfM-CAU_p3 TTTTGCAACTTTAGCTGCGG CTTGCGAGCTACCAGACTAC 45.0 55.0 57.9 57.8 501-502 bp 1.0 78.3
atpI-trnfM-CAU_p4 TTTTGCAACTTTAGCTGCGG GTTATGAGCCTTGCGAGCTA 45.0 50.0 57.9 57.8 510-511 bp 1.0 78.1
atpI-trnfM-CAU_p5 TTTTGCAACTTTAGCTGCGG TTATGAGCCTTGCGAGCTAC 45.0 50.0 57.9 57.8 509-510 bp 1.0 78.1