Hotspot

rpoA-rpoB

Genome · 19876-26398 · 6523 bp

Back to Gaultheria

Pi 0.00972

MarkerSeek score 58.70

Primer available yes

Label rpoA-rpoB

Feature Metadata

label_name: rpoA-rpoB
feature_type: igs
region: Genome
length_bp: 6523
variable_sites: 63
pi: 0.00972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rpoB_p1	CTCTTCTTTGATCTCCGCGA	ATCTTCTAGAGTCTCGGGCC	50.0	55.0	57.4	58.0	258 bp	1.0	78.8
rpoA-rpoB_p2	TCTCTTCTTTGATCTCCGCG	ATCTTCTAGAGTCTCGGGCC	50.0	55.0	57.4	58.0	259 bp	1.0	78.8
rpoA-rpoB_p3	TCTTTGATCTCCGCGATTGC	ATCTTCTAGAGTCTCGGGCC	50.0	55.0	58.7	58.0	254 bp	1.0	77.7
rpoA-rpoB_p4	CTCTTCTTTGATCTCCGCGA	TTCTTCGCCTTCATCGAGAC	50.0	50.0	57.4	57.7	231 bp	1.0	77.0
rpoA-rpoB_p5	TCTCTTCTTTGATCTCCGCG	TTCTTCGCCTTCATCGAGAC	50.0	50.0	57.4	57.7	232 bp	1.0	77.0