Hotspot

rpl32

SSC · 28450-28626 · 177 bp

Back to Gastrolobium

Pi 0.0508

MarkerSeek score 67.30

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 177
variable_sites: 9
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	CCACAGGAAACAGCAGAAGT	AAGCACGGAACCATGTGAAT	50.0	45.0	58.0	58.1	1494-1524 bp	1.0	84.7
rpl32_p2	CCACAGGAAACAGCAGAAGT	GATAAGATGCCATGCCGCTA	50.0	50.0	58.0	57.9	1116-1168 bp	1.0	84.5
rpl32_p3	CCACAGGAAACAGCAGAAGT	ACGGAACCATGTGAATGAGG	50.0	50.0	58.0	57.9	1490-1520 bp	1.0	84.5
rpl32_p4	CCACAGGAAACAGCAGAAGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1063-1115 bp	1.0	84.5
rpl32_p5	CCACAGGAAACAGCAGAAGT	CGAGAAAATTCCGCGAATCG	50.0	50.0	58.0	58.0	791 bp	0.5	59.7