Hotspot

ndhA

SSC · 34552-36938 · 2387 bp

Back to Gastrolobium

Pi 0.0328

MarkerSeek score 66.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2387
variable_sites: 76
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTGTTTAAGACAAGGTTTGAAT	TGGGAGAAGTTGATCGTTGA	27.3	45.0	52.4	56.5	2388-2450 bp	1.0	44.8
ndhA_p2	AGACAAGGTTTGAATATAGAAGATCA	TGGGAGAAGTTGATCGTTGA	30.8	45.0	56.2	56.5	2381-2443 bp	1.0	44.8
ndhA_p3	TTGTTTAAGACAAGGTTTGAAT	TGGGAGAAGTTGATCGTTGAA	27.3	42.9	52.4	57.2	2388-2450 bp	1.0	44.8
ndhA_p4	AGACAAGGTTTGAATATAGAAGATCA	TGGGAGAAGTTGATCGTTGAA	30.8	42.9	56.2	57.2	2381-2443 bp	1.0	44.8
ndhA_p5	TTGTTTAAGACAAGGTTTGAAT	ATGGGAGAAGTTGATCGTTGA	27.3	42.9	52.4	57.0	2389-2451 bp	1.0	44.8