Hotspot

rpl23-rpl2

IRa · 67856-67873 · 18 bp

Back to Gastrolobium

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	GTAAAGGGTAGAAGGGTGCG	CACTTGACTGTCTACGGCTC	55.0	55.0	58.0	58.0	198 bp	1.0	80.1
rpl23-rpl2_p2	GTAAAGGGTAGAAGGGTGCG	CCACAACGATGCTGTCCATA	55.0	50.0	58.0	58.0	245 bp	1.0	80.0
rpl23-rpl2_p3	TAAAGGGTAGAAGGGTGCGA	CACTTGACTGTCTACGGCTC	50.0	55.0	58.1	58.0	197 bp	1.0	79.8
rpl23-rpl2_p4	TAAAGGGTAGAAGGGTGCGA	CCACAACGATGCTGTCCATA	50.0	50.0	58.1	58.0	244 bp	1.0	79.7
rpl23-rpl2_p5	GTAAAGGGTAGAAGGGTGCG	GACTGTCTACGGCTCCATTG	55.0	55.0	58.0	58.1	193 bp	1.0	79.7