Hotspot

ndhG-ndhI

SSC · 33590-33985 · 396 bp

Back to Gastrolobium

Pi 0.084

MarkerSeek score 77.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 396
variable_sites: 31
pi: 0.084
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATGTAGCGATTCGGGCAAAT	TCAACAAACAATCCGAGCCA	45.0	45.0	58.0	58.0	855-871 bp	1.0	85.4
ndhG-ndhI_p2	ATGTAGCGATTCGGGCAAAT	CAACAAACAATCCGAGCCAC	45.0	50.0	58.0	57.9	854-870 bp	1.0	85.2
ndhG-ndhI_p3	GTGCTGCAGCTACAAAATGG	TCAACAAACAATCCGAGCCA	50.0	45.0	58.0	58.0	1015-1031 bp	1.0	84.8
ndhG-ndhI_p4	GTGCTGCAGCTACAAAATGG	CAACAAACAATCCGAGCCAC	50.0	50.0	58.0	57.9	1014-1030 bp	1.0	84.7
ndhG-ndhI_p5	AACAAACGCTCTTTCACCCT	TCAACAAACAATCCGAGCCA	45.0	45.0	57.9	58.0	1480 bp	0.5	59.4