Hotspot

rpl32-trnL-UAG

SSC · 28627-28894 · 268 bp

Back to Gastrolobium

Pi 0.0709
MarkerSeek score 69.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
268
variable_sites
19
pi
0.0709
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACAGCACTGAAAGCTTT AAGTTGCAAAGGCTTGGGTA 45.0 45.0 57.9 57.9 917-937 bp 1.0 84.0
rpl32-trnL-UAG_p2 TGGACAGCACTGAAAGCTTT AGTTGCAAAGGCTTGGGTAA 45.0 45.0 57.9 57.9 916-936 bp 1.0 84.0
rpl32-trnL-UAG_p3 TTGGACAGCACTGAAAGCTT AAGTTGCAAAGGCTTGGGTA 45.0 45.0 57.9 57.9 918-938 bp 1.0 84.0
rpl32-trnL-UAG_p4 TTGGACAGCACTGAAAGCTT AGTTGCAAAGGCTTGGGTAA 45.0 45.0 57.9 57.9 917-937 bp 1.0 84.0
rpl32-trnL-UAG_p5 TGGACAGCACTGAAAGCTTT AAGCACGGAACCATGTGAAT 45.0 45.0 57.9 58.1 840-860 bp 1.0 84.0