Hotspot

ndhF-rpl32

SSC · 27965-28449 · 485 bp

Back to Gastrolobium

Pi 0.0684

MarkerSeek score 76.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 485
variable_sites: 32
pi: 0.0684
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACACTCCAACCAATTCCC	CGAGAAAATTCCGCGAATCG	50.0	50.0	58.0	58.0	1168 bp	0.5	59.9
ndhF-rpl32_p2	CACACTCCAACCAATTCCCA	CGAGAAAATTCCGCGAATCG	50.0	50.0	58.0	58.0	1167 bp	0.5	59.9
ndhF-rpl32_p3	ACCAATCCCAACATTGCAGT	CGAGAAAATTCCGCGAATCG	45.0	50.0	58.0	58.0	1113 bp	0.5	59.8
ndhF-rpl32_p4	CCACAGGAAACAGCAGAAGT	CGAGAAAATTCCGCGAATCG	50.0	50.0	58.0	58.0	791 bp	0.5	59.7
ndhF-rpl32_p5	GCAACCGAACCGCAAAATAA	CGAGAAAATTCCGCGAATCG	45.0	50.0	57.9	58.0	1413 bp	0.5	59.5