Hotspot

rpl2-rpl23

IRb · 1584-1601 · 18 bp

Back to Gastrolobium

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CACTTGACTGTCTACGGCTC	GTAAAGGGTAGAAGGGTGCG	55.0	55.0	58.0	58.0	198 bp	1.0	80.1
rpl2-rpl23_p2	CCACAACGATGCTGTCCATA	GTAAAGGGTAGAAGGGTGCG	50.0	55.0	58.0	58.0	245 bp	1.0	80.0
rpl2-rpl23_p3	CACTTGACTGTCTACGGCTC	TAAAGGGTAGAAGGGTGCGA	55.0	50.0	58.0	58.1	197 bp	1.0	79.8
rpl2-rpl23_p4	CCACAACGATGCTGTCCATA	TAAAGGGTAGAAGGGTGCGA	50.0	50.0	58.0	58.1	244 bp	1.0	79.7
rpl2-rpl23_p5	GACTGTCTACGGCTCCATTG	GTAAAGGGTAGAAGGGTGCG	55.0	55.0	58.1	58.0	193 bp	1.0	79.7