Hotspot

ndhD

SSC · 113946-115087 · 1142 bp

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Pi 0.0173

MarkerSeek score 59.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1142
variable_sites: 77
pi: 0.0173
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1408-1885 bp	1.0	88.3
ndhD_p2	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.2	58.0	1407-1884 bp	1.0	88.0
ndhD_p3	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1398-1875 bp	1.0	87.9
ndhD_p4	TTCTGGAGCAGTATGGGCTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1399-1876 bp	1.0	87.9
ndhD_p5	AGGCATTCTTTCTGGAGCAG	GTACCCAATGTGTTCGAGCT	50.0	50.0	57.9	57.9	1524-2001 bp	1.0	87.5