Hotspot

ccsA-ndhD

SSC · 113683-113945 · 263 bp

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Pi 0.0339

MarkerSeek score 53.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 263
variable_sites: 31
pi: 0.0339
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	1129-1178 bp	0.882353	83.7
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	1128-1177 bp	0.882353	83.7
ccsA-ndhD_p3	TTTTACCCAAGGTTTCGCCA	TTGCATTGCCAGGAATGAGT	45.0	45.0	57.9	58.1	1248-1297 bp	0.882353	83.0
ccsA-ndhD_p4	AGGCATTCTTTCTGGAGCAG	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	858-907 bp	0.882353	83.0
ccsA-ndhD_p5	TGCAGCTCTTTTATGCGGAT	CATTGCCAGGAATGAGTGGT	45.0	50.0	58.0	58.2	1125-1174 bp	0.882353	83.0