Hotspot

rpl32-trnL-UAG

SSC · 111899-112486 · 588 bp

Back to Gastrochilus

Pi 0.0266

MarkerSeek score 69.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 588
variable_sites: 55
pi: 0.0266
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1169-1231 bp	1.0	87.9
rpl32-trnL-UAG_p2	GAGTCCGAAGCTTCTTTCGT	ATCCGCATAAAAGAGCTGCA	50.0	45.0	57.9	58.0	1903-2128 bp	1.0	87.8
rpl32-trnL-UAG_p3	GAGTCCGAAGCTTCTTTCGT	TCCGCATAAAAGAGCTGCAT	50.0	45.0	57.9	58.0	1902-2127 bp	1.0	87.8
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1288-1350 bp	1.0	87.7
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1289-1351 bp	1.0	87.6