Hotspot

rps8-rpl14

LSC · 82163-82403 · 241 bp

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Pi 0.0246

MarkerSeek score 62.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 19
pi: 0.0246
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	TGGTGCAATTGCTCGAGAAT	55.0	45.0	58.1	58.2	291-347 bp	1.0	86.9
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	ATTGCTCGAGAATTGCGACA	55.0	45.0	58.1	58.3	284-340 bp	1.0	86.2
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	TTGGTGCAATTGCTCGAGAA	55.0	45.0	58.1	58.4	292-348 bp	1.0	85.1
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	TTTGGTGCAATTGCTCGAGA	55.0	45.0	58.1	58.4	293-349 bp	1.0	85.1
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	TTTTGGTGCAATTGCTCGAG	55.0	45.0	58.1	57.6	294-350 bp	1.0	84.8