Hotspot

psbB-psbT

LSC · 75258-75621 · 364 bp

Back to Gastrochilus

Pi 0.0256

MarkerSeek score 70.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 35
pi: 0.0256
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	449-550 bp	1.0	73.1
psbB-psbT_p2	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	448-549 bp	1.0	70.9
psbB-psbT_p3	TCCAACTACAAGGAGACAGGT	TGGAACTTTAGGTGGTTCTCG	47.6	47.6	58.0	57.6	411-512 bp	1.0	70.5
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGAA	50.0	45.5	57.4	58.0	448-549 bp	1.0	68.3
psbB-psbT_p5	TCCAACTACAAGGAGACAGGT	GGAACTTTAGGTGGTTCTCGA	47.6	47.6	58.0	57.3	410-511 bp	1.0	68.3