Hotspot

psbM-trnD-GUC

LSC · 31066-32192 · 1127 bp

Back to Gastrochilus

Pi 0.0137

MarkerSeek score 64.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 64
pi: 0.0137
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	GTTCGAGCCCCGTCAGTC	45.0	66.7	58.3	60.1	1182-1223 bp	1.0	53.1
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	GTTCGAGCCCCGTCAGTC	45.0	66.7	57.6	60.1	1181-1222 bp	1.0	52.1
psbM-trnD-GUC_p3	AAATGAACAGCGCAGTAGCA	GTTCGAGCCCCGTCAGTC	45.0	66.7	58.5	60.1	1184-1225 bp	1.0	51.3
psbM-trnD-GUC_p4	AATGAACAGCGCAGTAGCAA	GTTCGAGCCCCGTCAGTC	45.0	66.7	58.5	60.1	1183-1224 bp	1.0	51.3
psbM-trnD-GUC_p5	GCGCAGTAGCAATAAAAGCA	GTTCGAGCCCCGTCAGTC	45.0	66.7	57.5	60.1	1175-1216 bp	1.0	50.8