Hotspot

rps16-trnQ-UUG

LSC · 6245-7126 · 882 bp

Back to Gastrochilus

Pi 0.0202
MarkerSeek score 65.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
882
variable_sites
59
pi
0.0202
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 556-965 bp 1.0 87.4
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 567-976 bp 1.0 86.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 560-969 bp 1.0 86.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 571-980 bp 1.0 85.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 562-971 bp 1.0 84.5