Hotspot

rps15

SSC · 127664-127936 · 273 bp

Back to Garuga

Pi 0
MarkerSeek score 29.60
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TCCTCGATTCATTCTTACATGA AAGGGGAGAGAGAGGATTGC 36.4 55.0 54.7 58.5 398 bp 1.0 40.0
rps15_p2 TCCTCGATTCATTCTTACATGA GGAGAGAGAGGATTGCGTTT 36.4 50.0 54.7 57.3 394 bp 1.0 40.0
rps15_p3 ACGAAAAGAAGTTCATCAAGATTCA AAGGGGAGAGAGAGGATTGC 32.0 55.0 57.5 58.5 366 bp 1.0 40.0
rps15_p4 TCCTCGATTCATTCTTACATGAA AAGGGGAGAGAGAGGATTGC 34.8 55.0 55.5 58.5 398 bp 1.0 40.0
rps15_p5 TTCCTCGATTCATTCTTACATGA AAGGGGAGAGAGAGGATTGC 34.8 55.0 55.5 58.5 399 bp 1.0 40.0