Hotspot

matK

LSC · 2179-3696 · 1518 bp

Back to Garuga

Pi 0.000439
MarkerSeek score 46.50
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1518
variable_sites
1
pi
0.000439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TTCGTATTGCCTGAACATTT CCTTGTTTTGACTGTATCGCA 35.0 42.9 53.5 56.8 1714 bp 1.0 40.0
matK_p2 TTCGTATTGCCTGAACATTT ACCTTGTTTTGACTGTATCGC 35.0 42.9 53.5 56.8 1715 bp 1.0 40.0
matK_p3 TTCGTATTGCCTGAACATTT CCTTGTTTTGACTGTATCGCAC 35.0 45.5 53.5 58.2 1714 bp 1.0 40.0
matK_p4 TTCGTATTGCCTGAACATTT CCCATGCATCATTTGATAACCC 35.0 45.5 53.5 57.7 1674 bp 1.0 40.0
matK_p5 TTCGTATTGCCTGAACATTT CCCCATGCATCATTTGATAACC 35.0 45.5 53.5 57.7 1675 bp 1.0 40.0