Hotspot

ccsA

SSC · 118672-119640 · 969 bp

Back to Garuga

Pi 0.000688
MarkerSeek score 46.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
1
pi
0.000688
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GGCGGCATAGCATCTTCTAA CAAAAGGTTCTCCTCGGCTT 50.0 50.0 57.8 58.1 1205 bp 1.0 77.9
ccsA_p2 GGCGGCATAGCATCTTCTAA TTCAAAAGGTTCTCCTCGGC 50.0 50.0 57.8 58.1 1207 bp 1.0 77.8
ccsA_p3 GGCGGCATAGCATCTTCTAA GGTTCTCCTCGGCTTATACC 50.0 55.0 57.8 57.2 1200 bp 1.0 72.2
ccsA_p4 TGGCGGCATAGCATCTTCTA CAAAAGGTTCTCCTCGGCTT 50.0 50.0 59.0 58.1 1206 bp 1.0 71.6
ccsA_p5 TGGCGGCATAGCATCTTCTA TTCAAAAGGTTCTCCTCGGC 50.0 50.0 59.0 58.1 1208 bp 1.0 71.6