Hotspot

ccsA-ndhD

SSC · 119641-119953 · 313 bp

Back to Garuga

Pi 0
MarkerSeek score 34.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
313
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGTGGCTTCTATGGGCTTTC CAGTGGACAAAGTCGAAGCT 50.0 50.0 57.9 58.1 454-455 bp 1.0 77.9
ccsA-ndhD_p2 TGTGGCTTCTATGGGCTTTC TCAGTGGACAAAGTCGAAGC 50.0 50.0 57.9 58.1 455-456 bp 1.0 77.9
ccsA-ndhD_p3 TTCCGCAATTGTGGCTTCTA CAGTGGACAAAGTCGAAGCT 45.0 50.0 57.8 58.1 463-464 bp 1.0 77.4
ccsA-ndhD_p4 TTCCGCAATTGTGGCTTCTA TCAGTGGACAAAGTCGAAGC 45.0 50.0 57.8 58.1 464-465 bp 1.0 77.4
ccsA-ndhD_p5 AATTCCGCAATTGTGGCTTC CAGTGGACAAAGTCGAAGCT 45.0 50.0 57.6 58.1 465-466 bp 1.0 76.0