Hotspot

rpl32-trnL-UAG

SSC · 117242-118479 · 1238 bp

Back to Garuga

Pi 0.000539
MarkerSeek score 46.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1238
variable_sites
1
pi
0.000539
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGTTGAAAGCTTTTTCGT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1361 bp 1.0 78.7
rpl32-trnL-UAG_p2 GGCGTTGAAAGCTTTTTCGT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1362 bp 1.0 78.7
rpl32-trnL-UAG_p3 GGCGTTGAAAGCTTTTTCGT GGATTCGAACCGAGATGCTT 45.0 50.0 58.2 58.1 1401 bp 1.0 78.3
rpl32-trnL-UAG_p4 GGCGTTGAAAGCTTTTTCGT TCGAACCGAGATGCTTTAGC 45.0 50.0 58.2 58.1 1397 bp 1.0 78.1
rpl32-trnL-UAG_p5 GGCGTTGAAAGCTTTTTCGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1371 bp 1.0 77.7