Hotspot

rps2

LSC · 31865-32575 · 711 bp

Back to Garnotia

Pi 0.00375

MarkerSeek score 52.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 5
pi: 0.00375
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCAAGCTATTTCGGCTCTTTCT	TCTACGCAGTTGCTCAAGAA	40.9	45.0	57.8	57.2	844 bp	1.0	56.3
rps2_p2	TCCGTAATGGTAAGACGAACA	TCTACGCAGTTGCTCAAGAA	42.9	45.0	56.8	57.2	800 bp	1.0	56.3
rps2_p3	TCAAGCTATTTCGGCTCTTTCT	ATCTACGCAGTTGCTCAAGA	40.9	45.0	57.8	57.0	845 bp	1.0	54.5
rps2_p4	TCCGTAATGGTAAGACGAACA	ATCTACGCAGTTGCTCAAGA	42.9	45.0	56.8	57.0	801 bp	1.0	54.5
rps2_p5	TCCGTAATGGTAAGACGAACAA	TCTACGCAGTTGCTCAAGAA	40.9	45.0	57.5	57.2	800 bp	1.0	53.9