Hotspot

ndhH

SSC · 116169-117350 · 1182 bp

Back to Garnotia

Pi 0.00931

MarkerSeek score 51.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 21
pi: 0.00931
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGGGTCAAAA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.6	58.2	1373 bp	1.0	76.4
ndhH_p2	CAGTAGGAGGGTCAAAATGGG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1370 bp	1.0	71.8
ndhH_p3	CCAGTAGGAGGGTCAAAATGG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1371 bp	1.0	71.8
ndhH_p4	CCCAGTAGGAGGGTCAAAATG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1372 bp	1.0	71.8
ndhH_p5	AGGAGGGTCAAAATGGGTAGA	TGCATTTTGATGAGCCTCGT	47.6	45.0	58.1	58.2	1366 bp	1.0	71.3