Hotspot

trnW-CCA-trnP-UGG

LSC · 65949-66087 · 139 bp

Back to Garnotia

Pi 0.0123

MarkerSeek score 55.20

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 3
pi: 0.0123
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	248-253 bp	1.0	80.9
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	247-252 bp	1.0	80.5
trnW-CCA-trnP-UGG_p3	ACCTACGACATCGGGTTTTG	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	240-245 bp	1.0	80.4
trnW-CCA-trnP-UGG_p4	TGGAATCACGCTCTGTAGGA	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	58.2	57.9	273-278 bp	1.0	80.2
trnW-CCA-trnP-UGG_p5	TGGAATCACGCTCTGTAGGA	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	58.2	58.2	272-277 bp	1.0	79.7