Hotspot

ndhC-trnV-UAC

LSC · 52139-53116 · 978 bp

Back to Garnotia

Pi 0.00803
MarkerSeek score 53.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
978
variable_sites
14
pi
0.00803
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 1023-1028 bp 1.0 49.6
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA AGCCCAATGCAGAAAAATGC 36.4 45.0 56.1 57.9 1116-1121 bp 1.0 49.3
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 1024-1029 bp 1.0 47.8
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 1022-1027 bp 1.0 45.6
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 1023-1028 bp 1.0 45.6