Hotspot

trnL-UAA-trnF-GAA

LSC · 49442-49827 · 386 bp

Back to Garnotia

Pi 0.0097

MarkerSeek score 55.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 7
pi: 0.0097
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	464-477 bp	1.0	78.9
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	481-494 bp	1.0	78.6
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	479-492 bp	1.0	78.5
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	480-493 bp	1.0	76.2
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	461-474 bp	1.0	75.7