Hotspot

ycf2

IRa · 144482-150838 · 6357 bp

Back to Garidella

Pi 0.00189

MarkerSeek score 42.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6357
variable_sites: 12
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CTGCAGGGTCCCAAATGAAT	TCGGTCGCTGAAGATCTTTG	50.0	50.0	58.1	58.0	766 bp	1.0	79.8
ycf2_p2	CTGCAGGGTCCCAAATGAAT	AAATAGAAGGCGCTCTGGTG	50.0	50.0	58.1	58.0	660 bp	1.0	79.7
ycf2_p3	CTGCAAAGAATTCTCGACGC	TCGGTCGCTGAAGATCTTTG	50.0	50.0	57.8	58.0	835 bp	1.0	79.6
ycf2_p4	CTGCAAAGAATTCTCGACGC	AAATAGAAGGCGCTCTGGTG	50.0	50.0	57.8	58.0	729 bp	1.0	79.5
ycf2_p5	CCGGGAAAAGCCATCTCTTT	TCGGTCGCTGAAGATCTTTG	50.0	50.0	58.2	58.0	1111 bp	1.0	79.4