Hotspot

ycf1

SSC · 122715-127772 · 5058 bp

Back to Garidella

Pi 0.00534

MarkerSeek score 41.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5058
variable_sites: 27
pi: 0.00534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAGAACCCCTGTCTAGAGC	GGAATCGTCCATTGCGCTAT	55.0	50.0	57.8	58.5	298 bp	1.0	76.0
ycf1_p2	GAGAACCCCTGTCTAGAGCT	GGAATCGTCCATTGCGCTAT	55.0	50.0	57.8	58.5	297 bp	1.0	76.0
ycf1_p3	TCCATAGCAAGAGAACCCCT	GGAATCGTCCATTGCGCTAT	50.0	50.0	57.7	58.5	307 bp	1.0	75.0
ycf1_p4	AACCCCTGTCTAGAGCTTCA	GGAATCGTCCATTGCGCTAT	50.0	50.0	57.7	58.5	294 bp	1.0	74.6
ycf1_p5	ACCCCTGTCTAGAGCTTCAA	GGAATCGTCCATTGCGCTAT	50.0	50.0	57.7	58.5	293 bp	1.0	74.6