Hotspot

ndhA

SSC · 118638-120721 · 2084 bp

Back to Garidella

Pi 0.00627

MarkerSeek score 53.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2084
variable_sites: 13
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGTTATGAATCTCCTGCGCC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	57.8	56.5	2120-2126 bp	1.0	66.8
ndhA_p2	GTTATGAATCTCCTGCGCCT	TGGGAGAAGTTGATCGTTGA	50.0	45.0	57.8	56.5	2119-2125 bp	1.0	66.8
ndhA_p3	AGTTATGAATCTCCTGCGCC	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	57.8	57.2	2120-2126 bp	1.0	64.8
ndhA_p4	GTTATGAATCTCCTGCGCCT	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	57.8	57.2	2119-2125 bp	1.0	64.8
ndhA_p5	GTTATGAATCTCCTGCGCCT	ATGGGAGAAGTTGATCGTTGA	50.0	42.9	57.8	57.0	2120-2126 bp	1.0	63.0