Hotspot

psbN-psbH

LSC · 74927-75052 · 126 bp

Back to Garidella

Pi 0.0238

MarkerSeek score 63.00

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 3
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GCGGTATATAAGGCGTACCC	ACTACCCTCAACGGTTTGTG	55.0	50.0	57.7	57.8	230 bp	1.0	76.8
psbN-psbH_p2	GCGGTATATAAGGCGTACCC	CTACCCTCAACGGTTTGTGT	55.0	50.0	57.7	57.8	229 bp	1.0	76.8
psbN-psbH_p3	GGCGTACCCGGTAAAACTTA	ACTACCCTCAACGGTTTGTG	50.0	50.0	57.6	57.8	219 bp	1.0	76.1
psbN-psbH_p4	GGCGTACCCGGTAAAACTTA	CTACCCTCAACGGTTTGTGT	50.0	50.0	57.6	57.8	218 bp	1.0	76.1
psbN-psbH_p5	TAAGGCGTACCCGGTAAAAC	CTACCCTCAACGGTTTGTGT	50.0	50.0	57.6	57.8	221 bp	1.0	76.1