Hotspot

psbB-psbT

LSC · 74445-74613 · 169 bp

Back to Garidella

Pi 0

MarkerSeek score 44.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTCAAGTGGAATTTGGAGC	TGATTCCTAGAGTCGAGACT	50.0	45.0	57.4	54.4	282-284 bp	1.0	46.8
psbB-psbT_p2	GCTCAAGTGGAATTTGGAGC	TCCTAGAGTCGAGACTAAGAGA	50.0	45.5	57.4	56.4	278-280 bp	1.0	46.4
psbB-psbT_p3	GCTCAAGTGGAATTTGGAGC	TCCTAGAGTCGAGACTAAGAGAA	50.0	43.5	57.4	57.1	278-280 bp	1.0	43.9
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TTCCTAGAGTCGAGACTAAGAGA	50.0	43.5	57.4	57.1	279-281 bp	1.0	43.9
psbB-psbT_p5	GCTCAAGTGGAATTTGGAGC	ATGATTCCTAGAGTCGAGACT	50.0	42.9	57.4	55.0	283-285 bp	1.0	43.8