Hotspot

ndhC-trnV-UAC

LSC · 50927-52004 · 1078 bp

Back to Garidella

Pi 0.00835

MarkerSeek score 63.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1078
variable_sites: 9
pi: 0.00835
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTCGTTAATTGGGGCCAA	AGTGCATGTTGGGTCTTTGA	50.0	45.0	58.4	57.6	1288-1293 bp	1.0	74.1
ndhC-trnV-UAC_p2	TAATTGGGGCCAAAACTCCG	AGTGCATGTTGGGTCTTTGA	50.0	45.0	58.4	57.6	1281-1286 bp	1.0	74.1
ndhC-trnV-UAC_p3	TGGTCCTTCGTTAATTGGGG	AGTGCATGTTGGGTCTTTGA	50.0	45.0	57.5	57.6	1292-1297 bp	1.0	73.6
ndhC-trnV-UAC_p4	CCTTCGTTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGA	50.0	50.0	58.4	57.5	1221-1226 bp	1.0	73.1
ndhC-trnV-UAC_p5	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGA	50.0	50.0	58.4	57.5	1214-1219 bp	1.0	73.1