Hotspot

trnS-GCU-trnR-UCU

LSC · 7049-8630 · 1582 bp

Back to Garidella

Pi 0.00696

MarkerSeek score 62.70

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1582
variable_sites: 11
pi: 0.00696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.0	56.7	1700-1760 bp	1.0	70.5
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.0	56.7	1699-1759 bp	1.0	70.5
trnS-GCU-trnR-UCU_p3	CTTTAGTCCACTCAGCCACC	TGGAAACAAATTGCGTCCAA	55.0	40.0	57.9	56.7	1690-1750 bp	1.0	69.7
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.2	56.7	1754-1814 bp	1.0	69.3
trnS-GCU-trnR-UCU_p5	TTTAGTCCACTCAGCCACCT	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.3	56.7	1689-1749 bp	1.0	68.4