Hotspot

rps16-trnQ-UUG

LSC · 5090-5682 · 593 bp

Back to Garidella

Pi 0.00675
MarkerSeek score 62.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
593
variable_sites
4
pi
0.00675
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCCTTCTCTTCGGGATCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 673-678 bp 1.0 78.8
rps16-trnQ-UUG_p2 TTCCTTCTCTTCGGGATCGA CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 677-682 bp 1.0 77.7
rps16-trnQ-UUG_p3 CTTCCTTCTCTTCGGGATCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.5 58.1 674-679 bp 1.0 75.9
rps16-trnQ-UUG_p4 TTCCTTCTCTTCGGGATCGA ACTCGGAGGTTCGAATCCTT 50.0 50.0 57.9 58.4 678-683 bp 1.0 75.8
rps16-trnQ-UUG_p5 CTTCCTTCTCTTCGGGATCG CTCGGAGGTTCGAATCCTTC 55.0 55.0 57.5 57.8 678-683 bp 1.0 74.8