Hotspot

ycf1

Genome · 125989-131604 · 5616 bp

Back to Gardneria

Pi 0.0041

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5616
variable_sites: 23
pi: 0.0041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGATGGGACGATTTAGCA	TGATATGTGGAGGAGTATTCC	50.0	42.9	57.5	53.9	337 bp	1.0	40.9
ycf1_p2	CGAGATGGGACGATTTAGCA	ACACAATCTTTTTGATATGTGGAGG	50.0	36.0	57.5	57.8	349 bp	1.0	40.9
ycf1_p3	CGAGATGGGACGATTTAGCA	TCTTTTTGATATGTGGAGGAGT	50.0	36.4	57.5	54.8	343 bp	1.0	40.9
ycf1_p4	CGAGATGGGACGATTTAGCA	TTGATATGTGGAGGAGTATTCC	50.0	40.9	57.5	54.8	338 bp	1.0	40.9
ycf1_p5	TCGAGATGGGACGATTTAGC	TGATATGTGGAGGAGTATTCC	50.0	42.9	57.2	53.9	338 bp	1.0	40.9