Hotspot

ndhG-ndhI

Genome · 120802-121278 · 477 bp

Back to Gardneria

Pi 0.02

MarkerSeek score 68.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: Genome
length_bp: 477
variable_sites: 9
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGCCCAGGT	AATCAAATTGCCCTGGGTCG	45.0	50.0	56.8	58.8	571-598 bp	1.0	69.2
ndhG-ndhI_p2	ATTGGCCCAGGTAAATCCAT	AATCAAATTGCCCTGGGTCG	45.0	50.0	56.8	58.8	563-590 bp	1.0	69.1
ndhG-ndhI_p3	GTATTGGCCCAGGTAAATCCA	AATCAAATTGCCCTGGGTCG	47.6	50.0	57.7	58.8	565-592 bp	1.0	68.0
ndhG-ndhI_p4	TGTATTGGCCCAGGTAAATCC	AATCAAATTGCCCTGGGTCG	47.6	50.0	57.7	58.8	566-593 bp	1.0	68.0
ndhG-ndhI_p5	AAATCATGTATTGGCCCAGGT	AATCAAATTGCCCTGGGTCG	42.9	50.0	57.6	58.8	572-599 bp	1.0	67.2