Hotspot

rpl32-trnL-UAG

Genome · 115474-116365 · 892 bp

Back to Gardneria

Pi 0.0114

MarkerSeek score 54.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 892
variable_sites: 10
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTGCAGCAAACAAAT	TTAGAAGATGACATGCCGCC	30.0	50.0	53.6	58.0	998-1011 bp	1.0	46.3
rpl32-trnL-UAG_p2	TTTTTGTGCAGCAAACAAAT	CGAGCACTGCTTCCTAAGAG	30.0	55.0	53.6	58.1	955-968 bp	1.0	46.1
rpl32-trnL-UAG_p3	TTTTTGTGCAGCAAACAAAT	CTCGAGCACTGCTTCCTAAG	30.0	55.0	53.6	58.1	957-970 bp	1.0	46.1
rpl32-trnL-UAG_p4	TTTTTGTGCAGCAAACAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.6	57.9	944-957 bp	1.0	45.8
rpl32-trnL-UAG_p5	TTTTTGTGCAGCAAACAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.6	57.8	937-950 bp	1.0	45.3