Hotspot

rps19-rpl2

Genome · 86774-86839 · 66 bp

Back to Gardneria

Pi 0.0312

MarkerSeek score 73.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 66
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CCGCGACCAAGTTACGATTA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	199-201 bp	1.0	80.1
rps19-rpl2_p2	CCGCGACCAAGTTACGATTA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.1	58.3	201-203 bp	1.0	78.7
rps19-rpl2_p3	CCGCGACCAAGTTACGATTA	CTGGGGTTATCCTGCACTTG	50.0	55.0	58.1	58.2	257-259 bp	1.0	78.6
rps19-rpl2_p4	CCGCGACCAAGTTACGATTA	TTTGATTCTTCGTCGCCGTA	50.0	45.0	58.1	57.6	200-202 bp	1.0	78.1
rps19-rpl2_p5	CCGCGACCAAGTTACGATTA	GAGCCCCGATTGGTAGAAAA	50.0	50.0	58.1	57.6	291-293 bp	1.0	77.1