Hotspot

clpP1-psbB

Genome · 74574-75038 · 465 bp

Back to Gardneria

Pi 0.00225

MarkerSeek score 61.40

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: Genome
length_bp: 465
variable_sites: 1
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	542-561 bp	1.0	81.1
clpP1-psbB_p2	CGCACTATACGTCAACCCAA	GGCCGGGATCATTCAATACA	50.0	50.0	58.0	57.7	574-593 bp	1.0	80.7
clpP1-psbB_p3	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	545-564 bp	1.0	79.3
clpP1-psbB_p4	TCCTCTCCAGGATTTCGGAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	513-532 bp	1.0	79.3
clpP1-psbB_p5	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	533-552 bp	1.0	79.1