Hotspot

psbE-petL

Genome · 67220-68386 · 1167 bp

Back to Gardneria

Pi 0.00686

MarkerSeek score 62.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1167
variable_sites: 8
pi: 0.00686
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAAACCA	50.0	42.9	57.9	57.3	1360-1362 bp	1.0	66.6
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAAACCA	50.0	42.9	57.8	57.3	1283-1285 bp	1.0	65.8
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGCTCAAACCA	45.0	42.9	58.2	57.3	1369-1371 bp	1.0	65.7
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGTCGTATCTTGCTCAAACCA	50.0	42.9	58.2	57.3	1374-1376 bp	1.0	65.6
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGTCGTATCTTGCTCAAACCA	50.0	42.9	57.8	57.3	1368-1370 bp	1.0	65.5