Hotspot

pafII-cemA

Genome · 62685-63564 · 880 bp

Back to Gardneria

Pi 0.00456

MarkerSeek score 61.60

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: Genome
length_bp: 880
variable_sites: 4
pi: 0.00456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	AAAAGCTGCCGAATTAGCCT	TGAGAGAGAAAGCCACCAGG	45.0	55.0	58.2	59.0	1008-1010 bp	1.0	71.0
pafII-cemA_p2	AAAAGCTGCCGAATTAGCCT	ATGAGAGAGAAAGCCACCAG	45.0	50.0	58.2	56.9	1009-1011 bp	1.0	70.6
pafII-cemA_p3	GCCGTTCCCTTAACTCGTAC	TGAGAGAGAAAGCCACCAGG	55.0	55.0	58.4	59.0	1061-1063 bp	1.0	69.4
pafII-cemA_p4	GCCGTTCCCTTAACTCGTAC	ATGAGAGAGAAAGCCACCAG	55.0	50.0	58.4	56.9	1062-1064 bp	1.0	68.9
pafII-cemA_p5	AAAAGCTGCCGAATTAGCCT	AATGAGAGAGAAAGCCACCAG	45.0	47.6	58.2	57.7	1010-1012 bp	1.0	68.4