Hotspot

trnE-UUC-trnT-GGU

Genome · 32739-33534 · 796 bp

Back to Gardneria

Pi 0.00503

MarkerSeek score 61.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 796
variable_sites: 4
pi: 0.00503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	937-938 bp	1.0	79.0
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	900-901 bp	1.0	78.9
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	916-917 bp	1.0	78.6
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	879-880 bp	1.0	78.5
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	913-914 bp	1.0	78.4