Hotspot

rpoB-trnC-GCA

Genome · 27944-29134 · 1191 bp

Back to Gardneria

Pi 0.00504

MarkerSeek score 61.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1191
variable_sites: 6
pi: 0.00504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1274 bp	1.0	71.6
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1279 bp	1.0	71.1
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1273 bp	1.0	70.5
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	57.9	50.0	58.0	58.4	1256 bp	1.0	69.4
rpoB-trnC-GCA_p5	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	1281 bp	1.0	69.2