Hotspot

atpH-atpI

Genome · 14538-15652 · 1115 bp

Back to Gardneria

Pi 0.00274

MarkerSeek score 62.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 1115
variable_sites: 3
pi: 0.00274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.0	57.9	1191-1201 bp	1.0	80.2
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TTTGCAACTTTAGCTGCAGC	55.0	45.0	58.1	57.9	1274-1284 bp	1.0	79.3
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TTTGCAACTTTAGCTGCAGC	55.0	45.0	57.9	57.9	1278-1288 bp	1.0	79.3
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.2	57.9	1192-1202 bp	1.0	79.0
atpH-atpI_p5	CCGGGTCCAATAGAAGCAAG	TTTGCAACTTTAGCTGCAGC	55.0	45.0	58.3	57.9	1233-1243 bp	1.0	77.8