Hotspot

trnH-GUG-psbA

Genome · 86-529 · 444 bp

Back to Gardneria

Pi 0.018
MarkerSeek score 58.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
Genome
length_bp
444
variable_sites
8
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 561-597 bp 1.0 79.8
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 575-611 bp 1.0 79.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 574-610 bp 1.0 79.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 576-612 bp 1.0 79.7
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCCTCTGGATCTAGCTGCTA 55.0 55.0 58.1 57.7 512-548 bp 1.0 77.8